Hi,
For finding absorption crosssection, why is it necessary that that the quantum yield of the fluorescence be constant for all the wavelengths in the absorption spectrum?
Using F = Abs X-section x I x Q
Is it because, to determine abs cross section at a wavelength, we can measure Fluorescence (F), Intensity of incident light (I) at the particular wavelength which will also be a known and hence determine Abs cross section at that wavelength using slope of the curve and the value of Q ? Q needs to be a constant and independent of wavelength, else we can't mathematically determine Abs xsection if Q has its own dependence on wavelength (and hence on abs xsection too). This can happen if we have a mixture of 2 fluorophores....perhaps there won't be a linear dependence of F on I anymore ?
Thanks a lot!
For finding absorption crosssection, why is it necessary that that the quantum yield of the fluorescence be constant for all the wavelengths in the absorption spectrum?
Using F = Abs X-section x I x Q
Is it because, to determine abs cross section at a wavelength, we can measure Fluorescence (F), Intensity of incident light (I) at the particular wavelength which will also be a known and hence determine Abs cross section at that wavelength using slope of the curve and the value of Q ? Q needs to be a constant and independent of wavelength, else we can't mathematically determine Abs xsection if Q has its own dependence on wavelength (and hence on abs xsection too). This can happen if we have a mixture of 2 fluorophores....perhaps there won't be a linear dependence of F on I anymore ?
Thanks a lot!